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- Title
Elucidating the Effect of Glycerol Concentration and C/N Ratio on Lipid Production Using Yarrowia lipolytica SKY7.
- Authors
Kuttiraja, Mathiazhakan; Douha, Ayed; Valéro, Jose; Tyagi, Rajeswar
- Abstract
The high demand for renewable energy and increased biodiesel production lead to the surplus availability of crude glycerol. Due to the above reason, the bio-based value addition of crude glycerol into various bioproducts is investigated; among them, microbial lipids are attractive. The present study was dedicated to find the optimal glycerol concentration and carbon/nitrogen (C/N) ratio to produce maximum lipid using Yarrowia lipolytica SKY7. The glycerol concentration (34.4 to168.2 g/L) and C/N ratio (25 to 150) were selected to investigate to maximize the lipid production. Initial glycerol concentration 112.5 g/L, C/N molar ratio of 100, and with 5 % v/ v inoculum supplementation were found to be optimum for biomass and lipid production. Based on the above optimal parameters, lipid concentration of 43.8 % w/ w with a biomass concentration of 14.8 g/L was achieved. In the case of glycerol concentration, the maximum Yp/s (0.192 g/g); Yx/s (0.43 g/g) was noted when the initial glycerol concentration was 112.5 g/L with C/N molar ratio 100 and inoculum volume 5 % v/ v. The glycerol uptake was also noted to increase with the increase in glycerol concentration. At low C/N ratio, the glycerol consumption was found to be high (79.43 g/L on C/N 25) whereas the glycerol consumption was observed to decrease when the C/N ratio was raised to 150 (40.8 g/L).
- Subjects
GLYCERIN; ALCOHOLS (Chemical class); BIODIESEL fuels; RENEWABLE energy sources; BIOMASS production
- Publication
Applied Biochemistry & Biotechnology, 2016, Vol 180, Issue 8, p1586
- ISSN
0273-2289
- Publication type
Academic Journal
- DOI
10.1007/s12010-016-2189-2