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Title

Microbial Degradation of Chlorogenic Acid by a Sphingomonas sp. Strain.

Authors

Ma, Yuping; Wang, Xiaoyu; Nie, Xueling; Zhang, Zhan; Yang, Zongcan; Nie, Cong; Tang, Hongzhi

Abstract

In order to elucidate the metabolism of chlorogenic acid by environmental microbes, a strain of Sphingomonas sp. isolated from tobacco leaves was cultured under various conditions, and chlorogenic acid degradation and its metabolites were investigated. The strain converting chlorogenic acid was newly isolated and identified as a Sphingomonas sp. strain by 16S rRNA sequencing. The optimal conditions for growth and chlorogenic acid degradation were 37 °C and pH 7.0 with supplementation of 1.5 g/l (NH)SO as the nitrogen source and 2 g/l chlorogenic acid as the sole carbon source. The maximum chlorogenic acid tolerating capability for the strain was 5 g/l. The main metabolites were identified as caffeic acid, shikimic acid, and 3,4-dihydroxybenzoic acid based on gas chromatography-mass spectrometry analysis. The analysis reveals the biotransformation mechanism of chlorogenic acid in microbial cells isolated from the environment.

Subjects

CHLOROGENIC acid; SPHINGOMONAS; MICROORGANISMS; METABOLITES; CAFFEIC acid

Publication

Applied Biochemistry & Biotechnology, 2016, Vol 179, Issue 8, p1381

ISSN

0273-2289

Publication type

Academic Journal

DOI

10.1007/s12010-016-2071-2

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