Results
Title

IL-17A Autoantibody Induced by Recombinant Mycobacterium smegmatis Expressing Ag85A-IL-17A Fusion Protein.

Authors

Chen, Ling; Guo, Sheng; Wu, Liangxia; Fan, Xiaoyong; Ma, Hui; Wu, Kang; Wu, Juan; Zhang, Jianhua

Abstract

Interleukin-17A is a newly described proinflammatory cytokine, which plays important roles in autoimmune diseases as well as asthma. In current work, we constructed a recombinant plasmid pMFA42S-Ag85a-IL-17a by inserting fusion gene Ag85a-IL-17a into shuttle vector pMFA42S, which was transformed to Mycobacterium smegmatis by electroporation to obtain recombinant M. smegmatis named rMS-Ag85a-IL-17a. The comparison of growth pattern between M. smegmatis and rMS-Ag85a-IL-17a suggested fusion gene had no significant influence on the growth of strains, and rMS-Ag85a-IL-17a expressed fusion protein Ag85A-IL-17A which had good immunogenicity revealed by Western blot. M. smegmatis and rMS-Ag85a-IL-17a were performed to intranasally immunize mice; then, antibody response in sera was detected by enzyme-linked immunosorbent assay. Our findings demonstrated that rMS-Ag85a-IL-17a could induce specific IL-17A autoantibody in mice, which laid the foundation for further study.

Subjects

INTERLEUKIN-17; MYCOBACTERIUM smegmatis; CHIMERIC proteins; AUTOANTIBODIES; ENZYME-linked immunosorbent assay; WESTERN immunoblotting

Publication

Applied Biochemistry & Biotechnology, 2015, Vol 176, Issue 7, p2018

ISSN

0273-2289

Publication type

Academic Journal

DOI

10.1007/s12010-015-1697-9