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- Title
IL-17A Autoantibody Induced by Recombinant Mycobacterium smegmatis Expressing Ag85A-IL-17A Fusion Protein.
- Authors
Chen, Ling; Guo, Sheng; Wu, Liangxia; Fan, Xiaoyong; Ma, Hui; Wu, Kang; Wu, Juan; Zhang, Jianhua
- Abstract
Interleukin-17A is a newly described proinflammatory cytokine, which plays important roles in autoimmune diseases as well as asthma. In current work, we constructed a recombinant plasmid pMFA42S-Ag85a-IL-17a by inserting fusion gene Ag85a-IL-17a into shuttle vector pMFA42S, which was transformed to Mycobacterium smegmatis by electroporation to obtain recombinant M. smegmatis named rMS-Ag85a-IL-17a. The comparison of growth pattern between M. smegmatis and rMS-Ag85a-IL-17a suggested fusion gene had no significant influence on the growth of strains, and rMS-Ag85a-IL-17a expressed fusion protein Ag85A-IL-17A which had good immunogenicity revealed by Western blot. M. smegmatis and rMS-Ag85a-IL-17a were performed to intranasally immunize mice; then, antibody response in sera was detected by enzyme-linked immunosorbent assay. Our findings demonstrated that rMS-Ag85a-IL-17a could induce specific IL-17A autoantibody in mice, which laid the foundation for further study.
- Subjects
INTERLEUKIN-17; MYCOBACTERIUM smegmatis; CHIMERIC proteins; AUTOANTIBODIES; ENZYME-linked immunosorbent assay; WESTERN immunoblotting
- Publication
Applied Biochemistry & Biotechnology, 2015, Vol 176, Issue 7, p2018
- ISSN
0273-2289
- Publication type
Academic Journal
- DOI
10.1007/s12010-015-1697-9