DNA-Binding Domain of DNA Ligase from the Thermophilic Archaeon Pyrococcus abyssi: Improving Long-Range PCR and Neutralization of Heparin's Inhibitory Effect.

Oscorbin, Igor; Boyarskikh, U.; Zakabunin, A.; Khrapov, E.; Filipenko, M.

The DNA-binding domain of the DNA ligase from Pyrococcus abyssi (PabDBD) was mapped and cloned into two expression vectors. The resulting 6X His-tagged proteins, with a predicted molecular mass of approximately 30 kDa, were overexpressed, purified using Ni-NTA resin, and biochemically characterized. Both PabDBD derivatives bound to double-stranded DNA fragments at the temperature range of 40-70 °C, and both were inactivated via heating at 95 °C for 15 min. Complexes of the PabDBD variants with either double- and single-stranded DNA fragments were less stable than the native DNA ligase of P. abyssi. Inclusion of the C-terminally 6X His-tagged PabDBD in the reaction mixture during long-range polymerase chain reaction (PCR) increased the efficacy of amplification and eliminated the inhibitory effect of heparin.

DNA-binding proteins; DNA ligases; PYROCOCCUS abyssi; PROTEIN expression; HEPARIN; THERMOPHILIC microorganisms; POLYMERASE chain reaction

Applied Biochemistry & Biotechnology, 2015, Vol 176, Issue 7, p1859

0273-2289

Academic Journal

10.1007/s12010-015-1683-2