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- Title
Improvement of Ansamitocin P-3 Production by Actinosynnema mirum with Fructose as the Sole Carbon Source.
- Authors
Li, Tinglan; Fan, Yuxiang; Nambou, Komi; Hu, Fengxian; Imanaka, Tadayuki; Wei, Liujing; Hua, Qiang
- Abstract
Ansamitocin P-3 (AP-3) is an active and potent anti-tumor maytansinoid, which is usually produced by Actinosynnema spp. In this study, the effects of different carbon sources on biomass and AP-3 production by Actinosynnema mirum were investigated. The results showed great biomass production behavior of A. mirum in glucose medium comparatively to other carbon sources. Interestingly, when fructose was used as the sole carbon source, the highest yield of AP-3 was obtained, which was about fourfold than that of strain cultured in glucose after 168 h. Further analysis conducted in regard to better understanding of such observations in glucose and fructose defined media showed that fructose improves AP-3 production through the stimulation of the key genes of the secondary metabolism pathways. It was concluded that fructose could be a potential carbon source for cost-effective production of AP-3 from an industrial point of view.
- Subjects
FRUCTOSE; BIOMASS production; CARBON; COST effectiveness; PHOSPHOFRUCTOKINASES
- Publication
Applied Biochemistry & Biotechnology, 2015, Vol 175, Issue 6, p2845
- ISSN
0273-2289
- Publication type
Academic Journal
- DOI
10.1007/s12010-014-1445-6