We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Cytotoxicity of Zinc Oxide Nanoparticles on Antioxidant Enzyme Activities and mRNA Expression in the Cocultured C2C12 and 3T3-L1 Cells.
- Authors
Pandurangan, Muthuraman; Veerappan, Muthuviveganandavel; Kim, Doo
- Abstract
The present study was aimed to investigate the dose-dependent effect of zinc oxide (ZnO) nanoparticles on antioxidant enzyme activities and messenger RNA (mRNA) expression in the cocultured C2C12 and 3T3-L1 cells. Coculturing experiments are 3D and more reliable compared to mono-culture (2D) experiment. Even though, there are several studies on ZnO nanoparticle-mediated cytotoxicity, but there are no studies on the effect of ZnO nanoparticle on antioxidant enzyme activities and mRNA expression in the cocultured C2C12 and 3T3-L1 cells. A cytotoxicity assay was carried out to determine the effect of ZnO nanoparticles on the C2C12 and 3T3-L1 cell viability. At higher concentration of ZnO nanoparticles, C2C12 and 3T3-L1 cells almost die. ZnO nanoparticles increased reactive oxygen species (ROS) and lipid peroxidation and reduced glutathione (GSH) levels in a dose-dependent manner in the C2C12 and 3T3-L1 cells. In addition, ZnO nanoparticles increased antioxidant enzyme activities and their mRNA expression in the C2C12 and 3T3-L1 cells. In conclusion, the present study showed that ZnO nanoparticles increased oxidative stress, antioxidant enzyme activities, and their mRNA expression in the cocultured C2C12 and 3T3-L1 cells.
- Subjects
PHYSIOLOGICAL effects of nanoparticles; ZINC oxide; ANTIOXIDANTS; MESSENGER RNA; OXIDATIVE stress; REACTIVE oxygen species; LIPID peroxidation (Biology); GLUTATHIONE
- Publication
Applied Biochemistry & Biotechnology, 2015, Vol 175, Issue 3, p1270
- ISSN
0273-2289
- Publication type
Academic Journal
- DOI
10.1007/s12010-014-1351-y