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- Title
Penicillium janthinellum: a Source of Efficient and High Levels of β-Glucosidase.
- Authors
Kaur, Amandeep; Chadha, B.
- Abstract
Penicillium janthinellum strain isolated from leaf litters of oak trees from montane alpine forests of Shivalik hills (India) produced high levels of β-glucosidase both during solid-state fermentation (796 units/gds) and shake flask cultures (65.3 units/ml). The peptide mass fingerprinting of the secretome showed a variety of glycosyl hydrolases. β-Glucosidase was purified and characterized to be a GH3 family member that had a molecular weight ( M) of 101 kDa and p I of 4.5. β-Glucosidase was optimally active at 60 °C at pH 5.0 but showed appreciable activity and thermostability under alkaline conditions (pH 9.0) also. β-Glucosidase activity was positively modulated in the presence of Mn ions. The enzyme preferentially catalyzed the hydrolysis of p-nitrophenol-β- d-glucopyranoside ( pNPG) but also recognized cellobiose as substrates. K and V for the hydrolysis of pNPG by β-glucosidase were calculated as 3.3 mM and 444 μmol min mg protein. Purified β-glucosidase showed transglycosylation activity in the presence of methanol as an acceptor molecule.
- Subjects
PENICILLIUM; GLUCOSIDASES; FERMENTATION; PEPTIDES; HYDROLASES; GLYCOSYLASES
- Publication
Applied Biochemistry & Biotechnology, 2015, Vol 175, Issue 2, p937
- ISSN
0273-2289
- Publication type
Academic Journal
- DOI
10.1007/s12010-014-1330-3