Title: Development of a Biological Protocol for Endotoxin Detection Using Quartz Crystal Microbalance (QCM).
Authors: Pérez-Lorenzo, E.; Zuzuarregui, A.; Arana, S.; Mujika, M.
Abstract: In this paper, a biological protocol for endotoxin detection has been developed and optimized by quartz crystal microbalance (QCM). The parameters involved in the formation of the self-assembled monolayer (SAM) have been analyzed, and a study of the pH of the ligand buffer has been performed in order to find the best condition for the ligand immobilization and, in consequence, for the endotoxin detection. The detection limit obtained with the characterized biological protocol corresponds to 1.90 μg/ml. The effectiveness of the optimized biological protocol has been analyzed by cyclic voltammetry analysis.
Subjects: ENDOTOXINS; QUARTZ crystal microbalances; ENCAPSULATION (Catalysis); CYCLIC voltammetry; POLYMYXIN B
Publication: Applied Biochemistry & Biotechnology, 2014, Vol 174, Issue 7, p2492
ISSN: 0273-2289
Publication type: Academic Journal
DOI: 10.1007/s12010-014-1198-2

Development of a Biological Protocol for Endotoxin Detection Using Quartz Crystal Microbalance (QCM).

Pérez-Lorenzo, E.; Zuzuarregui, A.; Arana, S.; Mujika, M.