Nymphaea rubra Ameliorates TNF-α-Induced Insulin Resistance via Suppression of c-Jun NH2-Terminal Kinase and Nuclear Factor-κB in the Rat Skeletal Muscle Cells.

Gautam, Sudeep; Rahuja, Neha; Ishrat, Nayab; Asthana, R.; Mishra, D.; Maurya, Rakesh; Jain, Swatantra; Srivastava, Arvind

In this work, we demonstrated insulin signaling and the anti-inflammatory effects by the chloroform fraction of ethanolic extract of Nymphaea rubra flowers in TNF-α-induced insulin resistance in the rat skeletal muscle cell line (L6 myotubes) to dissect out its anti-hyperglycemic mechanism. N. rubra enhances the GLUT4-mediated glucose transport in a dose dependent manner and also increases the tyrosine phosphorylation of both IR-β and IRS-1, and the IRS-1 associated PI-3 kinase activity in TNF-α-treated L6 myotubes. Moreover, N. rubra decreases Ser phosphorylation of IRS-1 by the suppression of JNK and NF-κB activation. In conclusion, N. rubra reverses the insulin resistance by the inhibition of c-Jun NH2-Terminal Kinase and Nuclear-κB.

NYMPHAEA; TUMOR necrosis factors; INSULIN resistance; IMMUNOSUPPRESSION; PROTEIN kinases; NF-kappa B; SKELETAL muscle; LABORATORY rats

Applied Biochemistry & Biotechnology, 2014, Vol 174, Issue 7, p2446

0273-2289

Academic Journal

10.1007/s12010-014-1192-8