Ferrocenylnaphthalene Diimide-Based Electrochemical Detection of Aberrant Methylation in hTERT Gene.

Sato, Shinobu; Saeki, Toshiro; Tanaka, Tomoki; Kanezaki, Yusuke; Hayakawa, Mana; Haraguchi, Kazuya; Kodama, Masaaki; Nishihara, Tatsuji; Tominaga, Kazuhiro; Takenaka, Shigeori

Since aberrant methylation at CpG sites is linked to the silencing of tumor suppressor genes, DNA methylation analysis is important for cancer diagnosis. We developed ferrocenylnaphthalene diimide (FND), which has two ferrocenyl moieties at the substituent termini, as an electrochemical indicator for hybridized DNA duplexes. In this study, we attempted to detect aberrant methylation of human telomerase reverse transcriptase gene ( hTERT), an efficient cancer marker, using FND-based hybridization coupled with electrochemical detection via a multi-electrode chip.

TUMOR suppressor genes; DNA methylation; CANCER diagnosis; METHYLATION; REVERSE transcriptase

Applied Biochemistry & Biotechnology, 2014, Vol 174, Issue 3, p869

0273-2289

Academic Journal

10.1007/s12010-014-1030-z