Kinetics of Enzymatic Hydrolysis of Olive Oil in Batch and Fed-batch Systems.

Cabral, Paloma; Filho, Arion; Voll, Fernando; Corazza, Marcos

This work reports experimental data, kinetic modeling, and simulations of enzyme-catalyzed hydrolysis of olive oil. This reaction was performed in batch system and an ordered-sequential Bi Bi model was used to model the kinetic mechanism. A fed-batch system was proposed and experimental data were obtained and compared to the simulated values. The kinetic model used was able to correlate the experimental data, in which a satisfactory agreement between the experimental data and modeling results was obtained under different enzyme concentration and initial free water content. Therefore, the modeling allowed a better understanding of the reaction kinetics and affords a fed-batch simulation for this system. From the results obtained, it was observed that the fed-batch approach showed to be more advantageous when compared to the conventional batch system.

OLIVE oil; HYDROLYSIS; BATCH reactors; CHEMICAL kinetics; ENZYMATIC analysis

Applied Biochemistry & Biotechnology, 2014, Vol 173, Issue 6, p1336

0273-2289

Academic Journal

10.1007/s12010-014-0943-x