We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Production of Xylooligosaccharides by Immobilized His-tagged Recombinant Xylanase from Penicillium occitanis on Nickel-Chelate Eupergit C.
- Authors
Driss, Dorra; Haddar, Anissa; Ghorbel, Raoudha; Chaabouni, Semia
- Abstract
Penicillium occitanis xylanase 2 expressed with a His-tag in Pichia pastoris, termed PoXyn2, was immobilized on nickel-chelate Eupergit C by covalent coupling reaction with a high immobilization yield up to 93.49 %. Characterization of the immobilized PoXyn2 was further evaluated. The optimum pH was not affected by immobilization, but the immobilized PoXyn2 exhibited more acidic and large optimum pH range (pH 2.0-4.0) than that of the free PoXyn2 (pH 3.0). The free PoXyn2 had an optimum temperature of 50 °C, whereas that of the immobilized enzyme was shifted to 65 °C. Immobilization increased both pH stability and thermostability when compared with the free enzyme. Time courses of the xylooligosaccharides (XOS) produced from corncob xylan indicated that the immobilized enzyme tends to use shorter xylan chains and to produce more xylobiose and xylotriose initially. At the end of 24-h reaction, XOS mixture contained a total of 21.3 and 34.2 % ( w/ w) of xylobiose and xylotriose with immobilized xylanase and free xylanase, respectively. The resulting XOS could be used as a special nutrient for lactic bacteria.
- Subjects
OLIGOSACCHARIDES; XYLANASES; PENICILLIUM; FUNGAL enzymes; IMMOBILIZED enzymes
- Publication
Applied Biochemistry & Biotechnology, 2014, Vol 173, Issue 6, p1405
- ISSN
0273-2289
- Publication type
Academic Journal
- DOI
10.1007/s12010-014-0932-0