Results
Title

Potential Xanthine Oxidase Inhibitory Activity of Endophytic Lasiodiplodia pseudotheobromae.

Authors

Kapoor, Neha; Saxena, Sanjai

Abstract

Xanthine oxidase is considered as a potential target for treatment of hyperuricemia. Hyperuricemia is predisposing factor for gout, chronic heart failure, atherosclerosis, tissue injury, and ischemia. To date, only two inhibitors of xanthine oxidase viz. allopurinol and febuxostat have been clinically approved for used as drugs. In the process of searching for new xanthine oxidase inhibitors, we screened culture filtrates of 42 endophytic fungi using in vitro qualitative and quantitative XO inhibitory assays. The qualitative assay exhibited potential XO inhibition by culture filtrates of four isolates viz. #1048 AMSTITYEL, #2CCSTITD, #6AMLWLS, and #96 CMSTITNEY. The XO inhibitory activity was present only in the chloroform extract of the culture filtrates. Chloroform extract of culture filtrate #1048 AMSTITYEL exhibited the highest inhibition of XO with an IC value of 0.61 μg ml which was better than allopurinol exhibiting an IC of 0.937 μg ml while febuxostat exhibited a much lower IC of 0.076 μg ml. Further, molecular phylogenetic tools and morphological studies were used to identify #1048 AMSTITYEL as Lasiodiplodia pseudotheobromae. This is the first report of an endophytic Lasiodiplodia pseudotheobromae from Aegle marmelos exhibiting potential XO Inhibitory activity.

Subjects

XANTHINE oxidase; ENDOPHYTIC fungi; HYPERURICEMIA; BIOLOGICAL assay; EXTRACTION (Chemistry); FUNGAL cultures; THERAPEUTICS

Publication

Applied Biochemistry & Biotechnology, 2014, Vol 173, Issue 6, p1360

ISSN

0273-2289

Publication type

Academic Journal

DOI

10.1007/s12010-014-0927-x