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- Title
Thermostable Hemicellulases of a Bacterium, Geobacillus sp. DC3, Isolated from the Former Homestake Gold Mine in Lead, South Dakota.
- Authors
Bergdale, Terran; Hughes, Stephen; Bang, Sookie
- Abstract
A thermophilic strain, Geobacillus sp. DC3, capable of producing hemicellulolytic enzymes was isolated from the 1.5-km depth of the former Homestake gold mine in Lead, South Dakota. The DC3 strain expressed a high level of extracellular endoxylanase at 39.5 U/mg protein with additional hemicellulases including β-xylosidase (0.209 U/mg) and arabinofuranosidase (0.230 U/mg), after the bacterium was grown in xylan for 24 h. Partially purified DC3 endoxylanase exhibited a molecular mass of approximately 43 kDa according to zymography with an optimal pH of 7 and optimal temperature of 70 °C. The kinetic constants, K and V, were 13.8 mg/mL and 77.5 μmol xylose/min·mg xylan, respectively. The endoxylanase was highly stable and maintained 70 % of its original activity after 16 h incubation at 70 °C. The thermostable properties and presence of three different hemicellulases of Geobacillus sp. DC3 strain support its potential application for industrial hydrolysis of renewable biomass such as lignocelluloses.
- Subjects
THERMOPHILIC bacteria; HEMICELLULOSE; GEOBACILLUS stearothermophilus; MICROBIOLOGY of extreme environments; ARABINOFURANOSIDASES; XYLANASES
- Publication
Applied Biochemistry & Biotechnology, 2014, Vol 172, Issue 7, p3488
- ISSN
0273-2289
- Publication type
Academic Journal
- DOI
10.1007/s12010-014-0784-7