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- Title
A β-Secretase (BACE1)-inhibiting C-Methylrotenoid Induced by Yeast Elicitation in Abronia nana Suspension Cultures.
- Authors
Kim, Sang-In; Yang, Eun-Ju; Park, Se-Hoon; Kim, Chang-Kil; Song, Kyung-Sik
- Abstract
Suspension cultures of Abronia nana were established to produce C-methylisoflavones. Treatment of the A. nana cultures with yeast elicitor induced boeravinone E ( 1), with maximum induction at 24 h after elicitor treatment. Of the biotic and abiotic elicitors tested, yeast extract gave the strongest induction of 1. The IC value of 1 against β-secretase (β-amyloid cleaving enzyme-1) was 5.57 μM. Other proteases such as trypsin, chymotrypsin, and elastase were not inhibited by concentrations up to 1.0 mM, indicating that inhibition of β-secretase was specific. 1 was noncompetitive in Dixon plot, and K i value was 3.79 μM.
- Subjects
SECRETASES; SECRETASE inhibitors; TRYPSIN; CHYMOTRYPSIN; ELASTASES
- Publication
Applied Biochemistry & Biotechnology, 2014, Vol 172, Issue 7, p3529
- ISSN
0273-2289
- Publication type
Academic Journal
- DOI
10.1007/s12010-014-0762-0