Antibacterial Activity of Synthetic Curcumin Derivatives: 3,5- Bis(benzylidene)-4-Piperidone (EF24) and EF24-Dimer Linked via Diethylenetriaminepentacetic Acid (EFDTPA).

Vilekar, Prachi; King, Catherine; Lagisetty, Pallavi; Awasthi, Vibhudutta; Awasthi, Shanjana

Curcumin is well known for its antimicrobial and anti-inflammatory properties. However, since systemic absorption and bioavailability of curcumin from gastrointestinal tract is considerably poor, synthetic curcuminoids are being developed as better alternatives. Two curcumin derivatives: 3,5- bis(benzylidene)-4-piperidone (EF24) and EF24-dimer linked via diethylenetriaminepentacetic acid (EFDTPA), were included in this study. We investigated the antibacterial activity of EF24 and EFDTPA against Gram-negative ( Escherichia coli) and Gram-positive ( Enterococcus faecalis, Staphylococcus aureus) bacteria. We also studied the effects of EF24 and EFDTPA on uptake and localization of pHrodo-labeled E. coli in the acidic compartments (phagolysosomes) of dendritic cells (DCs) under in vitro conditions. Our results demonstrate that treatment with EF24 and EFDTPA directly suppresses the bacterial growth. However, these compounds do not affect the bacterial uptake or localization in the DCs.

CURCUMIN; ESCHERICHIA coli biotechnology; STAPHYLOCOCCUS aureus; ANTIBACTERIAL agents; DENDRITIC cells; CURCUMINOIDS

Applied Biochemistry & Biotechnology, 2014, Vol 172, Issue 7, p3363

0273-2289

Academic Journal

10.1007/s12010-014-0741-5