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- Title
Purification and Characterization of RNA Allied Extracellular Tyrosinase from Aspergillus Species.
- Authors
Inamdar, Shrirang; Joshi, Swati; Bapat, Vishwas; Jadhav, Jyoti
- Abstract
Production of l-DOPA, an anti-Parkinson's drug, using biological sources is widely studied in which tyrosinase is known to play a vital role. Tyrosinase is an omnipresent type 3 copper enzyme participating in many essential biological functions. Understanding properties of tyrosinase is essential for developing useful tyrosinase-based applications. Hence, extracellular tyrosinase from Aspergillus flavus UWFP 570 was purified using ammonium sulphate precipitation and DEAE ion exchange chromatography up to 8.3-fold. Purified protein was a riboprotein in nature containing significant amount of RNA which was confirmed colorimetrically and by electrophoresis. Removal of RNA reduced the activity and altered the conformation of tyrosinase as suggested by spectroflurometric results. Optimum pH and temperature of this 140 kDa protein were 7 and 40 °C, respectively. Copper sulphate and magnesium chloride enhanced the activity whereas in contrast FeCl inhibited the activity completely. Purified tyrosinase transformed l-tyrosine (5 mM) to l-DOPA within 5 h.
- Subjects
ASPERGILLUS; PHENOL oxidase; RNA; DOPA; ION exchange chromatography; METHODS in Electrophoresis; ASPERGILLUS flavus; THERAPEUTICS
- Publication
Applied Biochemistry & Biotechnology, 2014, Vol 172, Issue 3, p1183
- ISSN
0273-2289
- Publication type
Academic Journal
- DOI
10.1007/s12010-013-0555-x