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- Title
Maximizing Hydrogen Production and Substrate Consumption by Escherichia coli WDHL in Cheese Whey Fermentation.
- Authors
Rosales-Colunga, Luis; Alvarado-Cuevas, Zazil; Razo-Flores, Elías; León Rodríguez, Antonio
- Abstract
Fermentative hydrogen production is strongly affected by pH. In order to maximize hydrogen production and substrate consumption in Escherichia coli Δ hycA, Δ lacI (WDHL) cheese whey fermentation, the influence of pH control at values of 5.5, 6, and 6.5 was studied in batch stirred-tank bioreactors. From the conditions evaluated, pH 6.5 was the best condition, at which the highest cumulative hydrogen production and yield (1.78 mol H/mol lactose) were obtained. Moreover, at this pH, all carbohydrates from the cheese whey were consumed, and a mix of ethanol and organic acids, mainly lactate, were produced from glucose, whereas galactose yielded acetate, ethanol, and succinate. Operating the reactor at pH 5.5 resulted in the highest maximum specific production rate, but smaller hydrogen yield because only glucose was metabolized and galactose was accumulated. At pH 6, not all cheese whey carbohydrates were consumed, and it was not favorable for hydrogen production. Lactose consumption and growth kinetics were not affected by the pH. The results show the importance of controlling pH to maximize hydrogen production and substrate consumption using cheese whey as substrate.
- Subjects
HYDROGEN production; BIOCHEMICAL substrates; ESCHERICHIA coli; FERMENTATION; WHEY; CHEESE
- Publication
Applied Biochemistry & Biotechnology, 2013, Vol 171, Issue 3, p704
- ISSN
0273-2289
- Publication type
Academic Journal
- DOI
10.1007/s12010-013-0394-9