Exon Array Analysis of Alternative Splicing of Genes in SOD1G93A Transgenic Mice.

Hu, Ming; Guo, Yansu; Chen, Huifang; Duan, Weisong; Li, Chunyan

Alternative splicing is a common strategy for creating functional diversities of proteins. While conventional identification of splice variants generally targets individual genes in amyotrophic lateral sclerosis, we present a novel exon-centric array that allows genome-wide identification of splice variants and concurrently provides analysis of gene expression. Compare 1 was asymptomatic SOD1G93A transgenic mice with nontransgenic littermates; compare 2 was symptomatic with asymptomatic transgenic mice. RT-PCR was performed to validate. Pathway and GO analysis were performed on abnormal genes. These findings could guide us to demonstrated the potential influence of mutant human CuZn-SOD1 and of splicing regulation in pathological processes.

GENETIC regulation; EXONS (Genetics); GENE expression; SPLIT genes; AMYOTROPHIC lateral sclerosis; TRANSGENIC mice

Applied Biochemistry & Biotechnology, 2013, Vol 170, Issue 2, p301

0273-2289

Academic Journal

10.1007/s12010-013-0155-9