Synthesis and Preliminary In Vitro Biological Evaluation of 5-Chloro-2-(Substituted Phenyl)Benzo[ d]Thiazole Derivatives Designed As Novel Antimelanogenesis Agents.

Ha, Young; Uehara, Yohei; Park, Daeui; Jeong, Hyoung; Park, Ji; Park, Yun; Lee, Ji; Lee, Hye; Song, Yu; Moon, Hyung; Chung, Hae

We describe the design, synthesis, and biological activities of 5-chloro-2-(substituted phenyl)benzo[ d]thiazole derivatives as novel tyrosinase inhibitors. Among them, 4-(5-chloro-2,3-dihydrobenzo[ d]thiazol-2-yl)-2,6-dimethoxyphenol ( MHY884) and 2-bromo-4-(5-chloro-benzo[ d]thiazol-2-yl)phenol ( MHY966) showed inhibitory activity higher than or similar to kojic acid, against mushroom tyrosinase. Therefore, we carried out kinetic studies on the two compounds with potent tyrosinase inhibitory effects. Kinetic analysis of tyrosinase inhibition revealed that all of these compounds are competitive inhibitors. MHY884 and MHY966 effectively inhibited tyrosinase activity and reduced melanin levels in B16 cells treated with α-melanocyte stimulating hormone (α-MSH). These data strongly suggest that the newly synthesized compounds MHY884 and MHY966 could suppress production of melanin via inhibition of tyrosinase activity.

FERTILIZATION in vitro; PHENYL compounds; THIAZOLES; PHENOL oxidase; MUSHROOMS

Applied Biochemistry & Biotechnology, 2012, Vol 168, Issue 6, p1416

0273-2289

Academic Journal

10.1007/s12010-012-9867-5