Title: Production of Mixed-Linkage Beta-Oligosaccharides from Lichenan Using Immobilized Bacillus licheniformis UEB CF Lichenase.
Authors: Chaari, Fatma; Blibech, Monia; Bhiri, Fatma; Maktouf, Sameh; Ellouz-Ghorbel, Raoudha; Ellouz-Chaabouni, Semia
Abstract: Lichenase from Bacillus licheniformis UEB CF was immobilized on Amberlite IR120 H. The immobilization yield and lichenase activity were 87 and 92.81 % of initial activity, respectively. The immobilized enzyme exhibited a shift in the optimal pH from 5.0 to 3.0, but the activity optimal temperature was not affected. The immobilized enzyme showed a residual activity of 50 % after five uses. It also exhibited high storage stability and retained 50 % of its initial activity after 120 days at 4 °C. The main hydrolysis products yielded from lichenan were trisaccharide and tetrasaccharide. The resulting mixed-linkage beta-oligosaccharides could be used as a special nutriment for lactic bacteria.
Subjects: OLIGOSACCHARIDES; LICHENASE; BACILLUS licheniformis; THERAPEUTIC immobilization; HYDROLYSIS
Publication: Applied Biochemistry & Biotechnology, 2012, Vol 168, Issue 3, p616
ISSN: 0273-2289
Publication type: Academic Journal
DOI: 10.1007/s12010-012-9804-7

Production of Mixed-Linkage Beta-Oligosaccharides from Lichenan Using Immobilized Bacillus licheniformis UEB CF Lichenase.

Chaari, Fatma; Blibech, Monia; Bhiri, Fatma; Maktouf, Sameh; Ellouz-Ghorbel, Raoudha; Ellouz-Chaabouni, Semia