Improvement of Hydrogen Productivity by Introduction of NADH Regeneration Pathway in Clostridium paraputrificum.

Lu, Yuan; Zhang, Chong; Zhao, Hongxin; Xing, Xin-Hui

To improve the hydrogen productivity and examine the hydrogen evolution mechanism of Clostridium paraputrificum, roles of formate in hydrogen evolution and effects of introducing formate-originated NADH regeneration were explored. The formate-decomposing pathway for hydrogen production was verified to exist in C. paraputrificum. Then NAD-dependent formate dehydrogenase FDH1 gene ( fdh1) from Candida boidinii was overexpressed, which regenerate more NADH from formate to form hydrogen by NADH-mediated pathway. With fdh1 overexpression, the hydrogen yield via NADH-involving pathway increased by at least 59 % compared with the control. Accompanied by the change of hydrogen metabolism, the whole cellular metabolism was redistributed greatly.

CLOSTRIDIUM toxins; BACILLACEAE; NAD (Coenzyme); CANDIDA boidinii; METABOLISM

Applied Biochemistry & Biotechnology, 2012, Vol 167, Issue 4, p732

0273-2289

Academic Journal

10.1007/s12010-012-9703-y