Metal-Based Biologically Active Compounds: Synthesis, Characterization, DNA Interaction, Antibacterial, Cytotoxic and SOD Mimic Activities.

Patel, Mohan; Patel, Chintan; Joshi, Hardik

The square pyramidal copper(II) complexes of N, O- donor ligand and ciprofloxacin have been synthesized. Synthesized complexes were characterized by physicochemical parameters like elemental analysis, electronic, FT-IR and LC-MS spectra. The complexes were screened for their antimicrobial activity against Gram( Ve), i.e. Staphylococcus aureus, Bacillus subtilis, and Gram(−Ve), i.e. Serratia marcescens, Pseudomonas aeruginosa and Escherichia coli, microorganisms in terms of minimum inhibitory concentration and colony-forming unit. To determine the binding mode of complexes with Herring Sperm DNA, absorption titration and viscosity measurement were employed. DNA cleavage activity was carried out by gel electrophoresis experiment using supercoiled form of pUC19 DNA. The complexes were tested for their superoxide dismutase mimic activity in terms of IC value. Synthesized complexes were also screened for their cytotoxicity using brine shrimp lethality assay method.

DNA-binding proteins; ANTIBODY-dependent cell cytotoxicity; LIGANDS (Biochemistry); METAL complexes; MOLECULAR biology; BINDING sites; HYDROGEN bonding

Applied Biochemistry & Biotechnology, 2013, Vol 169, Issue 4, p1329

0273-2289

Academic Journal

10.1007/s12010-012-0086-x