Sequence Analysis of KpnI Repeat Sequences to Revisit the Phylogeny of the Genus Carthamus L.

Mehrotra, Shweta; Goel, Shailendra; Sharma, Sarita; Raina, Soom; Rajpal, Vijay

Repetitive DNA sequences constitute a significant proportion of eukaryotic genomes. Knowledge about the distribution of repetitive DNA sequences is necessary in order to gain insights into the organization, evolution and behavior of eukaryotic genomes. Therefore, we used two repetitive DNA sequences pCt KpnI-I and pCt KpnI-II, earlier reported in Carthamus tinctorius L. to study the phylogeny and to revise the taxonomic status of the taxa belonging to the genus. The study unraveled two major lines within the genus Carthamus; one line included all the diploid taxa (2 n = 24) and the other line comprised the taxa with 2 n = 20 and the polyploid taxa (2 n = 44 and 64). The results of the present study will prove useful in molecular breeding for improving some targeted agronomic traits in genus Carthamus.

CARTHAMUS; REPEATED sequence (Genetics); PHYLOGENY; EUKARYOTIC genomes; NUCLEOTIDE sequence

Applied Biochemistry & Biotechnology, 2013, Vol 169, Issue 4, p1109

0273-2289

Academic Journal

10.1007/s12010-012-0063-4