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Title

Aldehyde Oxidase Activity and Stability in Water-Miscible Organic Solvents.

Authors

Rashidi, Mohammad-Reza; Dehghany, Moharam; Dehghan, Golamreza; Jouyban, Abolghasem; Faridi, Akram

Abstract

In this study, the catalytic activity of aldehyde oxidase was investigated in various water-miscible organic solvents for the first time. The enzyme was partially purified from rabbit liver, and its activity was determined in the absence/presence of nine hydro-organic mixtures. The effects of pH and temperature on the enzyme activity were also investigated. The activity was reduced in the presence of all nine organic solvents. However, in some cases, the residual activity remained almost unchanged throughout the incubation of enzyme at 35 °C for 24 h. Considering potential advantages of doing reactions in the presence of organic solvents, these results could be of value. The enzyme showed different behavior in the reaction solutions making it difficult to formulate results in a single comprehensive model. The results indicated that binding and cleavage of the substrate are influenced in the presence of organic solvents.

Subjects

ALDEHYDES; ORGANIC solvents; ENZYMES; ORGANIC compounds; SOLUTION (Chemistry)

Publication

Applied Biochemistry & Biotechnology, 2013, Vol 169, Issue 3, p901

ISSN

0273-2289

Publication type

Academic Journal

DOI

10.1007/s12010-012-0036-7

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