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- Title
Role of a Repeated Hexapeptide Motif GIHFAP Near C-terminus in Assembly, Stability, and Activity of 'HCH Dehydrochlorinase LinA'.
- Authors
Macwan, Ankit; Srivastava, Nidhi; Javed, Saleem; Kumar, Ashwani
- Abstract
Enzyme 'hexachlorocyclohexane (HCH) dehydrochlorinase LinA' mediates first step of aerobic microbial degradation of a chlorinated insecticide γ-HCH. The archetypal LinA-type1 consists of 156 amino acids that include a directly repeated hexapeptide motif GIHFAP at positions 141-146 and 148-153. Analysis of a series of LinA mutants, containing none, one, two, or three units of this repeated motif revealed that two units, as present in wild-type LinA, are required for its optimal activity and stability. Moreover, the presence of a bend in its secondary structure due to a proline residue that precedes the distal repeated unit contributes to enhanced LinA activity.
- Subjects
HEXACHLOROCYCLOHEXANES; INSECTICIDES; AMINO acids; PESTICIDES; HEXAPEPTIDES; ENZYMES
- Publication
Applied Biochemistry & Biotechnology, 2013, Vol 169, Issue 4, p1397
- ISSN
0273-2289
- Publication type
Academic Journal
- DOI
10.1007/s12010-012-0035-8