Results
Title

Activation of Kraft Lignin by an Enzymatic Treatment with a Versatile Peroxidase from Bjerkandera sp. R1.

Authors

Taboada-Puig, R.; Lú-Chau, T.; Moreira, M.; Feijoo, G.; Lema, J.

Abstract

Enzymatic lignin activation may be an environmentally friendly alternative to the use of chemicals in the production of wood fibers composites. Most studies on enzymatic activation of lignin for improving the adhesion of lignocellulosic products have been carried out using laccases. In this work, the use of a versatile peroxidase (VP) from the white-rot fungus Bjerkandera sp. (anamorph R1) for activating Kraft lignin was studied. The effect of enzyme dosage, incubation time, and HO addition profile on lignin activation was evaluated by quantifying the phenoxy radicals formed using electron paramagnetic resonance (EPR) spectroscopy. Two alternative enzymatic systems based on the use of VP (a two-stage and an enzymatic cascade system) were also assayed. At optimal conditions (dose of 15 U g and continuous addition of HO (5.24 μmol h) during 1 h) the content of phenoxy radicals was doubled as compared with an untreated control. Moreover, using the two-stage VP system, a lignin activation similar to that found at optimal conditions could be reached in a shorter time.

Subjects

ELECTRON paramagnetic resonance; LIGNINS; ENZYMES; LIGNOCELLULOSE; PEROXIDASE; PHENOXY radicals; MALONATES

Publication

Applied Biochemistry & Biotechnology, 2013, Vol 169, Issue 4, p1262

ISSN

0273-2289

Publication type

Academic Journal

DOI

10.1007/s12010-012-0023-z