We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Trehalose Has a Protective Effect on Human Brain-Type Creatine Kinase During Thermal Denaturation.
- Authors
Yang, Jiang-Liu; Mu, Hang; Lü, Zhi-Rong; Yin, Shang-Jun; Si, Yue-Xiu; Zhou, Sheng-Mei; Zhang, Fang; Hu, Wei-Jiang; Meng, Fan-Guo; Zhou, Hai-Meng; Zhang, Zi-Ping; Qian, Guo-Ying
- Abstract
We investigated the effects of trehalose on thermal inactivation and aggregation of human brain-type creatine kinase (hBBCK) in this study. In the presence of 1.0 M trehalose, the midpoint temperature of thermal inactivation ( T) of hBBCK increased by 4.6 °C, and the activation energy ( E) for thermal inactivation increased from 29.7 to 41.1 kJ mol. Intrinsic fluorescence spectra also showed an increase in the apparent transition temperature ( T) of hBBCK from 43.0 °C to 46.5 °C, 47.7 °C, and 49.9 °C in 0, 0.6, 0.8, and 1.2 M trehalose, respectively. In addition, trehalose significantly blocked the aggregation of hBBCK during thermal denaturation. Our results indicate that trehalose has potential applications as a thermal stabilizer and may aid in the folding of other enzymes in addition to hBBCK.
- Subjects
CREATINE kinase; DENATURATION of proteins; PROTEIN folding; BRAIN; PHYSIOLOGICAL effects of temperature
- Publication
Applied Biochemistry & Biotechnology, 2011, Vol 165, Issue 2, p476
- ISSN
0273-2289
- Publication type
Academic Journal
- DOI
10.1007/s12010-011-9266-3