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- Title
Screening and Identification of a Fungal β-Glucosidase and the Enzymatic Synthesis of Gentiooligosaccharide.
- Authors
Yongling Qin; Yunkai Zhang; Haiyan He; Jing Zhu; Guiguang Chen; Wei Li; Zhiqun Liang
- Abstract
fter screening with 0.1% esculoside and 0.03% FeCl, we identified from rotten wood a fungal isolate HML0366 that produces high amount of β-glucosidase. Phenotypic and rDNA internal transcribed spacer sequence analyses indicated that the isolate belongs to Aspergillus oryzae. The β-glucosidase produced by HML0366 had an activity of 128 U/g. high performance liquid chromatography analysis also demonstrated a high transglycosylation activity of the crude enzyme. The β-glucosidase was stable between pH 4-10 at 60 °C. A gentiobiose yield of 30.86 g/L was achieved within 72 h of the enzymatic reaction at pH 5 and 55 °C using 50% glucose as the substrate. For the first time, we report here the isolation of an A. oryzae strain producing β-glucosidase with high hydrolytic activities. The crude enzyme has a high transglycosylation activity, which enables the enzymatic synthesis of gentiooligosaccharides.
- Subjects
FERRIC chloride; GLUCOSIDASES; PHENOTYPES; ASPERGILLUS; GLUCOSE
- Publication
Applied Biochemistry & Biotechnology, 2011, Vol 163, Issue 8, p1012
- ISSN
0273-2289
- Publication type
Academic Journal
- DOI
10.1007/s12010-010-9105-y