Results
Title

Screening of Pectinase-Producing Microorganisms with Polygalacturonase Activity.

Authors

Zeni, Jamile; Lerin, Lindomar A.; Cansian, Rogério L.; Toniazzo, Geciane; De Oliveira, Débora; Valduga, Eunice; Cence, Karine; Grando, Camila Elis; Tiggerman, Lídia; Colet, Rosicler

Abstract

The aim of this work was to perform the screening of microorganisms, previously isolated from samples of agro-industrial waste and belonging to the culture collection of our laboratory, able to produce polygalacturonases (PG). A total of 107 microorganisms, 92 newly isolated and 15 pre-identified, were selected as potential producers of enzymes with PG activity. From these microorganisms, 20 strains were able to synthesize PG with activities above 3 U mL. After the kinetic study, the enzyme activity was increased up to 13 times and the microorganism identified as Aspergillus niger ATCC 9642 and the newly isolated W23, W43, and D2 ( Penicillium sp.) after 24 h of fermentation led to PG activities of 30, 41, 43, and 45 U mL, respectively. The RAPD analysis demonstrated that the selected strains differs genetically, indicating that no duplication of strains among them in the experiments for polygalacturonases production was verified.

Subjects

POLYGALACTURONASE; PENICILLIUM; ENZYMES; FERMENTATION; MICROORGANISMS

Publication

Applied Biochemistry & Biotechnology, 2011, Vol 163, Issue 3, p383

ISSN

0273-2289

Publication type

Academic Journal

DOI

10.1007/s12010-010-9046-5