Effects of Surfactant and Salt Species in Reverse Micellar Forward Extraction Efficiency of Isoflavones with Enriched Protein from Soy Flour.

Xiaoyan Zhao; Zhiyi Wei; Fangling Du; Junqing Zhu

Suitability of reverse micelles of anionic surfactant sodium bis(2-ethyl hexyl) sulfosuccinate (AOT) and sodium dodecyl sulfate (SDS), cationic surfactant hexadecyl trimethyl ammonium bromide (CTAB) and nonionic surfactant polyoxyethylene p-t-octylphenol (TritonX-100) in organic solvent isooctane for extraction of soy isoflavone-enriching proteins was investigated. The results showed that the order of combined isoflavone contents was SDS>CTAB>Triton X-100>AOT, while the order of protein recovery was SDS>AOT>TritonX-100>CTAB. As compared with ACN-HCl extraction, the total amount of isoflavones was lower than reverse micellar extraction. Ion strength was one of the important conditions to control extraction of isoflavone-enriching proteins with AOT reversed micelles. For the six salt systems, KNO, KCl, MgCl, CaCl, NaCl, and NaSO, extracted fraction of isoflavone-enriching proteins was measured. Salt solutions greatly influenced the extraction efficiency of isoflavones in an order of KNO>MgCl>CaCl>KCl>NaCl>NaSO, while protein in an order of MgCl>CaCl>NaCl>KNO>NaSO>KCl.

REVERSED micelles; ISOFLAVONES; SURFACE active agents; POLYETHYLENE glycol; PLANT proteins; ORGANIC solvents

Applied Biochemistry & Biotechnology, 2010, Vol 162, Issue 7, p2087

0273-2289

Academic Journal

10.1007/s12010-010-8984-2