We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
MFα Signal Peptide Enhances the Expression of Cellulase eg1 Gene in Yeast.
- Authors
Hong Zhu; Side Yao; Shilong Wang
- Abstract
Ethanol production from lignocellulose by recombinant yeast with high level expression of heterologous cellulase genes has been a major anticipation. The native secretion signal sequence of the cellulase endoglucanase I ( eg1) gene was replaced by Saccharomyces cerevisiae mating factor α prepro-leader sequence (MFα). The transformants containing native secretion signal ( Y1) and MFα secretion signal ( Y2) were characterized with respect to gene expression and growth on cellulose substrate. Increased enzyme activity and cellulose utilization were observed. The enzyme activity of Y2 was 0.084 U/ml, 61.5% higher than Y1 (0.052 U/ml). The sufficiency parameter ( S value) was raised from 0.6 to 0.84. MFα signal peptide was more efficient than the native signal peptide of eg1 gene, suggesting that signal peptide replacement is an efficient way to enhance the cellulase expression level in yeast, for cellulose-derived ethanol production.
- Subjects
GENE expression; SIGNAL peptidases; CELLULASE; YEAST; LIGNOCELLULOSE
- Publication
Applied Biochemistry & Biotechnology, 2010, Vol 162, Issue 3, p617
- ISSN
0273-2289
- Publication type
Academic Journal
- DOI
10.1007/s12010-009-8880-9