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Title

Solid-Phase Synthesis of a Biotin Derivative and its Application to the Development of Anti-Biotin Antibodies.

Authors

Papasarantos, Ilias; Klimentzou, Persefoni; Koutrafouri, Vassiliki; Anagnostouli, Maria; Zikos, Christos; Paravatou-Petsotas, Maria; Livaniou, Evangelia

Abstract

A biotin derivative, namely biotin–aminocaproic acid–lysine (BAL), was synthesized with solid-phase chemistry, conjugated to a carrier-protein, and used for rabbit immunization. The aminocaproic acid–lysine “long-arm” was used in order to project the biotin-hapten above the carrier-protein surface. Lysine was selected due to its Nε-amino group, through which BAL was conjugated to the carrier-protein. BAL was synthesized on a commercially available resin with the Fmoc-solid-phase strategy; this has simplified the experimental procedure, overcome the need for intermediate purification steps, and led to a final product of high purity, with high yield. The anti-BAL antibodies recognized free biotin, as shown with an in-house-developed ELISA, in which biotin conjugated to a synthetic “lysine–dendrimer” was used to coat the ELISA microwells. In immunocytology and Western-blot experiments, the anti-BAL antibodies led to similar results with those obtained with streptavidin. Synthetic derivatives of hapten molecules that can be easily prepared with solid-phase chemistry, such as BAL, may be used for the development of specific antibodies for the corresponding hapten.

Subjects

SOLID-phase synthesis; BIOTIN; CARRIER proteins; IMMUNOGLOBULINS; HAPTENS; LABORATORY rabbits; IMMUNIZATION

Publication

Applied Biochemistry & Biotechnology, 2010, Vol 162, Issue 1, p221

ISSN

0273-2289

Publication type

Academic Journal

DOI

10.1007/s12010-009-8870-y

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