Role of Toll-Like Receptor 3, RIG-I, and MDA5 in the Expression of Mesothelial IL-8 Induced by Viral RNA.

Wörnle, Markus; Sauter, Matthias; Kastenmüller, Kathrin; Ribeiro, Andrea; Mussack, Thomas; Ladurner, Roland; Sitter, Thomas

Interleukin-8 (IL-8) is a chemokine that has been shown to be a potent chemoattractant for polymorphonuclear neutrophils from the vascular compartment into the pleural space during infectious pleural effusions. Mesothelial cells express the viral receptors Toll-like receptor 3 (TLR3), RIG-I, and MDA5. Activation of these receptors by viral RNA exemplified by poly (I:C) RNA leads to a time- and dose-dependent increase of mesothelial IL-8 synthesis. To show the specific effect of viral receptors, knockdown experiments with short interfering RNA specific for TLR3, RIG-I and MDA5 were performed. This novel finding of functional expression of these viral sensors on human mesothelial cells may indicate a novel link between viral infections and mesothelial inflammation and indicates a pathophysiologic role of viral receptors in these processes.

CHEMOKINES; MESOTHELIUM; NEUTROPHILS; RNA; PATHOLOGICAL physiology

Applied Biochemistry & Biotechnology, 2010, Vol 160, Issue 4, p1179

0273-2289

Academic Journal

10.1007/s12010-009-8643-7