Determination of Cytochrome c in Human Serum and Pharmaceutical Injections Using Flow Injection Chemiluminescence.

Xin Li; Houyong Liu; Xili He; Zhenghua Song

It was found that the complex of cytochrome c (Cyt c) and hydrogen peroxide could significantly catalyze the chemiluminescence (CL) reaction from luminol-hydrogen peroxide, and a sensitive, rapid, and simple CL procedure was proposed for the determination of Cyt c in a flow injection system for the first time. The increment of CL intensity was linear over the concentration of Cyt c ranging from 5 to 700 ng ml-1, with a detection limit of 2 ng ml-1 (3σ). At a flow rate of 2.0 ml min-1, a complete analytical process could be performed in 30 s with a relative standard deviation of less than 4.0%. The proposed method was applied successfully for the assay of Cyt c in pharmaceutical injections and human serum, and the recoveries were from 98.0% to 108.8% and 92.5% to 109.0%. The possible mechanism of Cyt c enhanced CL reaction was also discussed.

CYTOCHROME c; CHEMILUMINESCENCE; HYDROGEN peroxide; FLOW injection analysis; CATALYSIS

Applied Biochemistry & Biotechnology, 2010, Vol 160, Issue 4, p1065

0273-2289

Academic Journal

10.1007/s12010-009-8598-8