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- Title
Screening of Food Grade Lipases to be Used in Esterification and Interesterification Reactions of Industrial Interest.
- Authors
de Paula, Ariela Veloso; Morais Nunes, Gisele Fátima; Silva, Josiane de Lourdes; de Castro, Heizir Ferreira; dos Santos, Júlio César
- Abstract
Seven food grade commercially available lipases were immobilized by covalent binding on polysiloxane-polyvinyl alcohol (POS-PVA) hybrid composite and screened to mediate reactions of industrial interest. The synthesis of butyl butyrate and the interesterification of tripalmitin with triolein were chosen as model reactions. The highest esterification activity (240.63 µM/g min) was achieved by Candida rugosa lipase, while the highest interesterification yield (31%, in 72 h) was achieved by lipase from Rhizopus oryzae, with the production of about 15 mM of the triglycerides C50 and C52. This lipase also showed a good performance in butyl butyrate synthesis, with an esterification activity of 171.14 µM/g min. The results demonstrated the feasibility of using lipases from C. rugosa for esterification and R. oryzae lipase for both esterification and interesterification reactions.
- Subjects
SILOXANES; POLYVINYL alcohol; LIPASES; ESTERIFICATION; CANDIDA
- Publication
Applied Biochemistry & Biotechnology, 2010, Vol 160, Issue 4, p1146
- ISSN
0273-2289
- Publication type
Academic Journal
- DOI
10.1007/s12010-009-8578-z