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- Title
Improving Enzymatic Production of Ginsenoside Rh<sub>2</sub> from Rg<sub>3</sub> by using Nonionic Surfactant.
- Authors
Jin-Huan Su; Jian-He Xu; Zhi-Long Wang
- Abstract
In this study, several nonionic surfactants were tried to improve the enzymatic hydrolysis of ginsenoside Rg3 into Rh2 which was catalyzed at 50 °C and pH 5.0 by a crude glucosidase extracted from Fusarium sp. ECU2042. Among the biocompatible nonionic surfactants, polyethylene glycol 350 monomethyl ether was shown to be the best. After optimizing some influencing factors on the reaction, the conversion of Rg3 (5 g/l) with 10 g/l crude enzyme reached almost 100% in the presence of the nonionic surfactant (7.5%, w/v), which was 25% higher than that in buffer without any surfactant. Furthermore, the enzyme stability was affected faintly by the surfactant.
- Subjects
SURFACE active agents; HYDROLYSIS; FUSARIUM; CATALYSIS; GLUCOSIDASES; POLYETHYLENE glycol
- Publication
Applied Biochemistry & Biotechnology, 2010, Vol 160, Issue 4, p1116
- ISSN
0273-2289
- Publication type
Academic Journal
- DOI
10.1007/s12010-009-8570-7