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- Title
Improved Production of (R)-1-phenyl-1,2-ethanediol by a Codon-optimized R-specific Carbonyl Reductase from Candida parapsilosis in Escherichia coli.
- Authors
Rongzhen Zhang; Yan Xu; Yawei Geng; Shanshan Wang; Ying Sun; Rong Xiao
- Abstract
Abstract An R-specific carbonyl reductase from Candida parapsilosis (CprCR) catalyzes the transformation of (R)-1-phenyl-1,2-ethanediol from 2-hydroxyacetophenone. The gene rcr coding CprCR contains a few codons rarely used by Escherichia coli. In order to improve chiral alcohol production, three codon variants Δ24, aRCR, and mRCR of CprCR were designed through truncation of 4–27 bp disorder sequence at the 5′-terminus or/and adaption of nine rare codons. The effects of codon optimization on enzyme activity, protein production, and biotransformation were studied. Among these three types, the disorder sequence-truncated and rare codon-adapted variant mRCR presents the highest enzyme activity. When compared with CprCR, mRCR showed an increase of 35.6% in the total activity of cell-free extracts. The specific activity of mRCR presented similar increase in the cell-free extract with purified protein, which suggested that the codon optimization caused positive effect on protein productivity of variant enzyme. When microbial cells concentration was 30% (w/v), the molar conversion yield and enantiomeric excess of the mRCR variant reached 86.4% and 93.6%, which were increased 36.5% and 15.8% than those of wild-type at a high substrate concentration of 5 g/L. The work will supply a new method for improving chiral alcohol preparation with codon engineered microorganisms.
- Subjects
ETHYLENE glycol; CARBONYL reductase; CANDIDA; ESCHERICHIA coli; YEAST; GENETIC code; ENZYMES
- Publication
Applied Biochemistry & Biotechnology, 2010, Vol 160, Issue 3, p868
- ISSN
0273-2289
- Publication type
Academic Journal
- DOI
10.1007/s12010-009-8528-9