Results
Title

Improvement of Steroid Biotransformation with Hydroxypropyl-β-Cyclodextrin Induced Complexation.

Authors

Liting Zhang; Min Wang; Yanbing Shen; Yinhu Ma; Jianmei Luo

Abstract

Abstract  The inclusion complexes induced by cyclodextrins and its derivates have been shown previously to enhance the biotransformation of hydrophobic compounds. Using hydroxypropyl-β-cyclodextrin (HP-β-CD; 20% w/v), the water solubility of cortisone acetate increased from 0.039 to 7.382 g L−1 at 32 °C. The solubilization effect of HP-β-CD was far superior to dimethylformamide (DMF) and ethanol. The dissolution rate also significantly increased in the presence of HP-β-CD. The enzymatic stability of Δ1-dehydrogenase from Arthrobacter simplex TCCC 11037 was not influenced by the increasing concentrations of HP-β-CD contrary to the organic cosolvents which negatively influenced in the order DMF > ethanol. The activity inhibition effect caused by HP-β-CD was not so conspicuous as ethanol and DMF. Inactivation constants of ethanol, DMF, and HP-β-CD were 5.832, 4.541, and 1.216, respectively. The inactivation energy (E a) was in the order of HP-β-CD (55.1 kJ mol−1) > ethanol (39.9 kJ mol−1) > DMF (37.1 kJ mol−1).

Subjects

BIOTRANSFORMATION (Metabolism); STEROIDS; CYCLODEXTRINS; SURFACE chemistry; DEHYDROGENASES; ARTHROBACTER; HYDROPHOBIC surfaces

Publication

Applied Biochemistry & Biotechnology, 2009, Vol 159, Issue 3, p642

ISSN

0273-2289

Publication type

Academic Journal

DOI

10.1007/s12010-008-8499-2