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- Title
Recombination System Based on Cre α Complementation and Leucine Zipper Fusions.
- Authors
Azadeh Seidi; Masayasu Mie; Eiry Kobatake
- Abstract
Abstract In a previous study, a recombination system based on the α complementation of cre recombinase and protein transduction was established. This system relied on the transient expression of the inactive, self-excisable C-terminal (β) and the transduction of the N-terminal (α) cre fragments to cells as a purified protein. This recombination system potentially results in a less invasive and more controllable cre recombination in mammalian cells. In this study, we have employed a more efficient complementation triggering sequence using more than only the overlapping amino acids to help the α and β fragments reassociate. In order to increase the association efficiency of the complementing fragments of cre recombinase, we chose to use a fusion of cre fragments to a self-heterodimerizing pair of proteins to trigger their binding and thus increase the efficiency of the restored enzymatic activity. For this purpose, the leucine zipper motifs (bJun and bFos) of the AP-1 transcription were fused to cre fragments (α and β, respectively). This resulted in an increased reassociation efficiency of the fragments and a two times more efficient recombination system compared with the previous study.
- Subjects
GENETIC recombination; LEUCINE zippers; CELLULAR signal transduction; CELL separation; MAMMAL cytology; DNA-binding proteins; AMINO acids
- Publication
Applied Biochemistry & Biotechnology, 2009, Vol 158, Issue 2, p334
- ISSN
0273-2289
- Publication type
Academic Journal
- DOI
10.1007/s12010-008-8409-7