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Title

An α-Glucuronidase Enzyme Activity Assay Adaptable for Solid Phase Screening.

Authors

Charles Lee; Kurt Wagschal; Rena Kibblewhite-Accinelli; William Orts; George Robertson; Dominic Wong

Abstract

Abstract  Glucuronic acid is a common chemical moiety that decorates the xylan polymer of hemicellulose. This chemical substituent impairs both enzymatic and acidic hydrolysis of xylosidic bonds. The α-glucuronidase enzyme hydrolyzes the 1,2-linked glucuronic acid from the terminal, non-reducing xylose of xylo-oligosaccharides. There are relatively few α-glucuronidase genes in the public databases. We have developed an assay with commercially available reagents that can be used to search DNA libraries for α-glucuronidase genes in a high-throughput, solid phase activity screen.

Subjects

GLUCURONIDASE; ENZYMOLOGY; SOLID-phase biochemistry; HEMICELLULOSE; HYDROLYSIS; CHEMICAL bonds; HIGH throughput screening (Drug development); BIOLOGICAL assay

Publication

Applied Biochemistry & Biotechnology, 2009, Vol 155, Issue 1-3, p11

ISSN

0273-2289

Publication type

Academic Journal

DOI

10.1007/s12010-008-8408-8

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