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- Title
Fatty Acid Profiling During Microbial Lipid Production Under Varying p O<sub>2</sub> and Impeller Tip Speeds.
- Authors
Syed Ahmed; Sudheer Singh; Ashok Pandey; Sanjit Kanjilal; Rachapudi Prasad
- Abstract
Abstract  The fatty acid profile study was undertaken to study the effect of impeller tip speed-associated shear stress and dissolved oxygen saturation (DO) on the fatty acid composition variation and on total lipid content of the cells. The study was undertaken in a 5-l stirred tank bioreactor using Mucor sp. RRL001. To study the interaction of parameters and their effects, a central composite design was used. The fatty acid profiling during the course of study suggested that oleic acid and palmitic acid were two major components with their composition varying between 34â47% and 29â39.1%, respectively, of the total lipid content. The GLA content varied between 3% and 9% of the total lipid. The lipid profile study also revealed the presence of a minor amount of fatty acids of chain length C:12, C:20, C:22, and C:24. The modeling of lipid accumulation suggested that it follows a quadratic model with both impeller tip speed (pâ=â0.0166) and dissolved oxygen concentration (pâ=â0.0098) following the quadratic order of effect. The fermenter run based on the optimum production zone in response surface plot resulted in the maximum 4.8 g lâ1 lipid compared with the model-predicted value of 4.49 g lâ1. The present study suggests that dissolved oxygen saturation is a more significant contributor to total lipid accumulation. However, the study also suggests that the fatty acid profile of fungal lipid is not directly associated with the shear stress or oxygen availability in Mucor sp. RRL001.
- Subjects
MICROBIAL lipids; FATTY acids; IMPELLERS; OLEIC acid; PALMITIC acid; BIOREACTORS; OXYGEN
- Publication
Applied Biochemistry & Biotechnology, 2008, Vol 151, Issue 2/3, p599
- ISSN
0273-2289
- Publication type
Academic Journal
- DOI
10.1007/s12010-008-8261-9