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- Title
Effects of Organic Solvents on Immobilized Lipase in Pectin Microspheres.
- Authors
L. Costas; V. Bosio; A. Pandey
- Abstract
Abstract  Lipase from Brevibacillus agri 52 was found stable up to 90% diethylenglycol (DEG), glycerol (GLY), and 1,2 propanediol (1,2 PRO) at 37 °C for 1 h and the stability was reduced only approximately 20% after 12 h incubation, but in 40% dimethylsulfoxide (DMSO), lipase activity was stable only for 1 h. Inhibition of the biocatalysts with dimethylformamide (DMF) was detected at 20% solvent concentration. In water immiscible systems, the stability of lipase in n-hexane, n-tetradecane and n-heptane resembles the water activity, but in the presence of isobutanol, 1-hexanol, and butylbutirate, the stability was significantly reduced. Lipase 52 precipitates in the presence of 50% acetone or ethanol/water mixtures, but enzymatic activity was partially recovered by adding 20% GLY, DEG, 1,2 PRO, or DMSO to the reaction mixture. Furthermore, by increasing DEG in 70% DMF/DEG mixtures, the lipase activity was protected. Encapsulation of lipase in pectin gels cross-linked with calcium ions brings three to four times more enzymatic activity in 70% water miscible organic solvents compared to aqueous systems.
- Subjects
MICROSPHERES; PECTINS; ORGANIC solvents; LIPASES; DIMETHYL sulfoxide; ENZYME inhibitors
- Publication
Applied Biochemistry & Biotechnology, 2008, Vol 151, Issue 2/3, p578
- ISSN
0273-2289
- Publication type
Academic Journal
- DOI
10.1007/s12010-008-8233-0