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- Title
Production and Purification of a Solvent-Resistant Esterase from Bacillus licheniformis S-86.
- Authors
Sebastián Torres; Mario BaigorÃ; Ashok Pandey
- Abstract
Abstract  New thermophilic and organic-solvent-tolerant Bacillus licheniformis S-86 strain is able to produce two active and solvent-stable esterases. Production of type I and II esterases was substantially enhanced when oils and surfactants were supplied as carbon sources. Grape oil (0.1% v/v) and Tween 20 to 60 (0.1% v/v) had enhanced enzyme production between 1.6- and 2.2-folds. Type II esterase was purified to homogeneity in a five-step procedure. This esterase was purified 76.7-fold with a specific activity of 135 U mgâ1. Molecular mass of the enzyme was estimated to be 38.4 kDa by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Type II esterase was active mostly on esters with short acyl chains, which allowed to classify the enzyme as a carboxylesterase with a K m of 80.2 mmol lâ1 and a V max of 256.4 μmol minâ1 mgâ1 for p-nitrophenyl acetate. Also, B. licheniformis S-86 type II esterase displayed activity in presence of water-miscible organic solvents at 50% concentration and stability after 1-h incubation.
- Subjects
ESTERASES; BACILLUS (Bacteria); ENZYMES; CHEMICAL synthesis; CHEMICAL purification; SURFACE active agents; ORGANIC solvents; POLYACRYLAMIDE gel electrophoresis
- Publication
Applied Biochemistry & Biotechnology, 2008, Vol 151, Issue 2/3, p221
- ISSN
0273-2289
- Publication type
Academic Journal
- DOI
10.1007/s12010-008-8181-8