Abstract A commercial strain of Saccharomyces cerevisiae was used for the production of ethanol by fermentation of cashew apple juice. Growth kinetics and ethanol productivity were calculated for batch fermentation with different initial sugar (glucose fructose) concentrations. Maximal ethanol, cell, and glycerol concentrations were obtained when 103.1 g L−1 of initial sugar concentration was used. Cell yield (Y X/S) was calculated as 0.24 (g microorganism)/(g glucose fructose) using cashew apple juice medium with 41.3 g L−1 of initial sugar concentration. Glucose was exhausted first, followed by fructose. Furthermore, the initial concentration of sugars did not influence ethanol selectivity. These results indicate that cashew apple juice is a suitable substrate for yeast growth and ethanol production.