Optimization of Oligosaccharide Synthesis from Cellobiose by Dextransucrase.

Misook Kim; Donal Day

Abstract  There is a growing market for oligosaccharides as sweeteners, prebiotics, anticariogenic compounds, and immunostimulating agents in both food and pharmaceutical industries. Interest in novel carbohydrate-based products has grown because of their reduced toxicity and low immune response. Cellobiose is potentially valuable as a nondigestible sugar. The reaction of cellobiose, as an acceptor with a sucrose as a donor, catalyzed by a dextransucrase from Leuconostoc mesenteroides B-512FMCM, produced a series of cellobio-oligosaccharides. This production system was optimized using a Box–Behnken experimental design for 289 mM of sucrose and 250 mM of cellobiose and 54 U of the enzyme at pH 5.2 and 30 °C, to produce maximum yields of oligosaccharide.

OLIGOSACCHARIDES; PHARMACEUTICAL industry; SUCROSE; SWEETENERS

Applied Biochemistry & Biotechnology, 2008, Vol 148, Issue 1-3, p189

0273-2289

Academic Journal

10.1007/s12010-007-8042-x