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- Title
Optimization of Oligosaccharide Synthesis from Cellobiose by Dextransucrase.
- Authors
Misook Kim; Donal Day
- Abstract
Abstract There is a growing market for oligosaccharides as sweeteners, prebiotics, anticariogenic compounds, and immunostimulating agents in both food and pharmaceutical industries. Interest in novel carbohydrate-based products has grown because of their reduced toxicity and low immune response. Cellobiose is potentially valuable as a nondigestible sugar. The reaction of cellobiose, as an acceptor with a sucrose as a donor, catalyzed by a dextransucrase from Leuconostoc mesenteroides B-512FMCM, produced a series of cellobio-oligosaccharides. This production system was optimized using a Box–Behnken experimental design for 289 mM of sucrose and 250 mM of cellobiose and 54 U of the enzyme at pH 5.2 and 30 °C, to produce maximum yields of oligosaccharide.
- Subjects
OLIGOSACCHARIDES; PHARMACEUTICAL industry; SUCROSE; SWEETENERS
- Publication
Applied Biochemistry & Biotechnology, 2008, Vol 148, Issue 1-3, p189
- ISSN
0273-2289
- Publication type
Academic Journal
- DOI
10.1007/s12010-007-8042-x