9-hydroxy-10-oxo-12(Z),15(Z)-octadecadienoic acid (KODA) enhances adventitious root redifferentiation from Swertia japonica callus.

Kawakami, Hiroko; Yokoyama, Mineyuki; Takagi, Kazuteru; Ogawa, Shigeyuki; Hara, Kojiro; Komine, Masashi; Yamamoto, Yoshikazu

The stress-induced α-ketol derivative of linolenic acid, identified in Lemna paucicostata as 9-hydroxy-10-oxo-12(Z),15(Z)-octadecadienoic acid (KODA), is involved in the enhancement of flower formation and the breaking of dormancy. Swertia japonica is an important bitter stomachic, and its adventitious roots contain xanthones, which are antioxidant compounds. Here, we demonstrate that KODA enhanced redifferentiation of adventitious roots from calli of S. japonica. Moreover, the effect of KODA was confirmed in liquid culture and the redifferentiation of adventitious roots was increased more than in solid culture. Conversely, KODA suppressed the biosynthesis of xanthone diglycoside, which suggests that KODA maintained the roots in an immature state under our culture conditions.

LEMNA paucicostata; ABIOTIC stress; ROOTING of plant cuttings; SWERTIA; XANTHONE; CALLUS (Botany)

In Vitro Cellular & Developmental Biology Plant, 2015, Vol 51, Issue 2, p201

1054-5476

Academic Journal

10.1007/s11627-015-9676-y