Growth regulator-induced betacyanin accumulation and dopa-4,5-dioxygenase ( DODA) gene expression in euhalophyte Suaeda salsa calli.

Shu-Zhen Zhao; Hong-Zhao Sun; Ye Gao; Na Sui; Bao-Shan Wang

Suaeda salsa calluses cultured in darkness for 28 d were transferred to Murashige and Skoog (MS) media containing various growth regulators under white light conditions for 10 d to investigate cell growth, betacyanin accumulation, and expression of dopa-4,5-dioxygenase ( DODA). Callus growth was markedly promoted when 0.2 mg·L 2,4-D and 0.5 mg·L 6-BA were added to the MS medium. Surprisingly, of the auxins tested, IAA had no effect on betacyanin content, but 2,4-D strongly decreased betacyanin content. Betacyanin content was positively correlated with 6-BA concentrations in the range of 0.1-2.0 mg·L. DODA mRNA levels were consistent with the response of betacyanin content to exogenous growth regulators. These results suggest that betacyanin metabolism in S. salsa calluses is regulated under white light conditions by growth regulators through the regulation of genes such as DODA that are involved in betacyanin synthesis.

PLANT regulators; PLANT cell development; CALLUS (Botany); AUXIN; PLANT growth regulation

In Vitro Cellular & Developmental Biology Plant, 2011, Vol 47, Issue 3, p391

1054-5476

Academic Journal

10.1007/s11627-011-9339-6