In vitro propagation of Sandersonia aurantiaca Hook using thidiazuron.

Deroles, Simon C.; Seelye, John F.; Javellana, John; Mullan, Andrew C.

A reliable in vitro propagation system was developed for the cut flower crop sandersonia ( Sandersonia aurantiaca Hook). Explants comprised immature embryos from developing seeds and meristems from sprouting tubers. MS media supplemented with a range of thidiazuron (TDZ) concentrations lead to the formation of multiple shoot clusters. To overcome the deleterious effect of long-term exposure to TDZ concentrations, a multistage protocol of 4-weekly cycles was used. This comprised exposure to a high TDZ (3.0 mg l−1) medium for a single cycle to induce proliferation, followed by one or two cycles of low TDZ (0.3 mg l−1) media to allow for the development of shoot structures, and then a rest phase on growth-regulator free medium for one cycle prior to the return to a medium with high TDZ. Shoot clusters on growth-regulator free media formed minitubers (10–12 weeks), which sprouted, either in vitro, or ex vitro, with the resulting plants successfully acclimatised in the greenhouse.

PLANT propagation; PLANT cuttings; PLANT physiology; PLANT cells & tissues; MERISTEMS; PLANT stems

Plant Cell, Tissue & Organ Culture, 2010, Vol 102, Issue 1, p115

0167-6857

Academic Journal

10.1007/s11240-010-9705-9