Regeneration of transformed plants from hairy roots of Plumbago indica.

Gangopadhyay, Moumita; Chakraborty, Dipjyoti; Bhattacharyya, Somnath; Bhattacharya, Sabita

Spontaneous shoot regeneration was observed from Agrobacterium rhizogenes-induced hairy roots of Plumbago indica when these were incubated in liquid MS medium for a period of 3 weeks under continuous light. Insertion of the rolB gene in putative transformed plants was confirmed by PCR and sequencing. Transformed plants grown for a period of 1 week on solid MS medium containing 0.5 mg l−1 6-benzyladenine and then transplanted to growth regulator-free medium showed better overall growth than control plants. Transformed plants had a higher root bio-biomass and an increased plumbagin content relative to non-transformed plants.

PLANT shoots; REGENERATION (Botany); AGROBACTERIUM; GRAPHITE; POLYMERASE chain reaction; BIOMASS

Plant Cell, Tissue & Organ Culture, 2010, Vol 102, Issue 1, p109

0167-6857

Academic Journal

10.1007/s11240-010-9702-z